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Illumina Microarrays
Illumina生物晶片具超高密度探針數且應用廣泛,從發現應用到常規篩查,
微陣列是用於變異檢測的有價值的工具。
Illumina生物晶片可提供加速研究所需的可信賴數據質量。隨著基因組信息被納入精密醫學計劃,臨床和轉化研究,藥理學,消費者篩選和農業應用中,我們將繼續開發微陣列以滿足各種研究需求。 無論您每天要運行幾千個樣本,生物晶片都可以幫助您有效地分析。
Methylation BeadChip (Human / Mouse)
HumanMethylation EPIC晶片提供超過850,000個探針(probe)來進行DNA甲基化(human methylation)之偵測。 此晶片一次可進行8個樣品的實驗,為目前進行表觀遺傳學(Epigenetics)研究最多人選用之平台,也是目前提供最多探針之晶片。
生物分析項目
- Methylation profile Normalization by Illumina GenomeStudio
- Differentially methylated probe set identification
(根據客戶提供sample比較的資訊) - Sample clustering by methylation profile
(clustering方法為hierarchical clustering)
Basic analysis
- SSN+BMIQ normalization
- M-value calculation
- Differentially methylated probe set and gene set identification
(根據客戶提供sample比較的資訊) - Gene Ontology and pathway analysis according to differentially methylated genes
- Customized analysis based on customers’ requirements
Advanced analysis
實驗與分析流程
sample collection
DNA extraction
scanning chips
Bioinformatics Analysis
Transform image to digit data
Expression Profile
Pathway Analysis
Case and control analysis
Differentially expressed gene
gene ontology analysis
樣品需求
A260/230 >1.0
更多晶片產品資訊請見Illumina原廠官網:https://www.illumina.com/products/by-type/microarray-kits.html
Genotyping BeadChip
Omni Array 系列 提供最佳且最廣泛的基因型探針,可偵測通用(commom)及較罕見(rare)的基因型。Omni 2.5和Omni 5晶片的差異主要在於探針(probe)的數量以及每片晶片所能進行的樣品數目。 Omni 2.5的探針數為250萬,而Omni 5的探針數為500萬。Omni 2.5每片晶片可進行的sample數量為8個,而Omni 5每片晶片可進行的sample數量為4個。
生物分析項目
- Genotyping 結果報告
- Allele frequency 結果報告
Basic analysis
- Risk calculation for SNP (根據客戶提供sample的內容)
- Haplotype 分析 (根據客戶提供sample的內容)
- Customized analysis based on customers’ requirements
Advanced analysis
實驗與分析流程
sample collection
DNA extraction
scanning chips
Bioinformatics Analysis
Transform image to digit data
Case and control analysis
Differentially expressed gene
樣品需求
A260/230 >1.0
更多晶片產品資訊請見Illumina原廠官網:https://www.illumina.com/products/by-type/microarray-kits.html
主要服務規格
類型 | 晶片名稱 | 探針數 | 最低檢體數量 | 檢體類別 | 總量 | 濃度 | OD260/280 | OD260/230 | 備註 |
---|---|---|---|---|---|---|---|---|---|
Methylation | EPIC | 850K | 8 samples per chip | DNA | >1.5ug | 50ng/ul | 1.8-2.2 | ≥1.0 | gDNA>2kb |
BSDNA | NA | >7.5ng/ul | NA | NA | 體積>12ul | ||||
Mouse | 285K | 12 samples per chip | DNA | >1.5ug | 50ng/ul | 1.8-2.2 | ≥1.0 | gDNA>2kb | |
BSDNA | NA | >7.5ng/ul | NA | NA | 體積>12ul | ||||
Genotyping | omni 5 | >4.2M | 16 samples | DNA | >1ug | 50ng/ul | 1.8-2.2 | ≥1.0 | gDNA>2kb |
onmi 2.5 | 2.5M | 16 samples | DNA | >1ug | 50ng/ul | 1.8-2.2 | ≥1.0 | gDNA>2kb | |
ASA | 600K | 48 samples | DNA | >1ug | 50ng/ul | 1.8-2.2 | ≥1.0 | gDNA>2kb |
更多晶片產品資訊請見Illumina原廠官網:https://www.illumina.com/products/by-type/microarray-kits.html