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Gut Health
益生菌

Genetic Testing of the gastrointestinal microbiota.

Product manual
The profiling of human gut microbiota can depict personal health information that is hidden in the gut microbiomes.

Majority of the microbes that live within humans are located in the gut. These microbes are very important for nutrient metabolism and absorption as well as immune system regulation. the gut is also a very complex neural network which often refers to as the Second Brain. gut microbes are also related to numerous physical and mental diseases according to the current research.

The genetic testing using next-generation sequencing (NGS) technology can profile bacteria in your gut. This data is then compared with more than 150,000 strains of bacteria in the global bacteria database to confirm the type and proportion of microbes in your intestine.

This test report analyzes the proportion of beneficial and harmful bacteria which are associated with constipation, diarrhea, and gastrointestinal nutrient absorption capacity. After analyzing your gut microbiota, we categorized them into five categories; Excessive harmful microbe, gastrointestinal sensitive, functional degradation, nutritional disorders, daily health maintenance which provides you an appropriate health maintenance program using probiotics.

腸胃 益生菌

Non-invasive home sampling

The detection of human gut microflora is sampled at home in a non-invasive and simple manner. Genetic information regards to the gut microbiome can be obtained through the simple collection of feces by sampling rods.

Technology and Products
基因 保健

Feces Sample

gene report

High-throughput sequencing

16S rRNA variable segment

DNA 檢測

Bioinformatics analysis

  • Since feces can reflect the conditions in the intestine, the test is performed by the collection of fecal samples. Bacteria DNA in the fecal sample is then extracted.
  • Sequencing the 16S rRNA variable segments of bacterial DNA through high-throughput sequencing technology.
  • Performing bioinformatics analysis on the sequencing results to profile microbiome in the gut. By combining the sequencing results, HGT’s proprietary reference database and curated information from international journal literature, gut health status can therefore be depicted from the gut microbiome analysis.
保健 益生菌
  • HGT: ISO17025 laboratory; certification laboratory of the largest sequencing instrument manufacturer in the world, Illumina (USA); the first certified laboratory in Taiwan for the latest third-generation sequencer manufacturer, Oxford Nanopore Technologies (ONT, UK).
  • Sampling rod: British medical material manufacturer (MWE), preserve gut microbe sample effectively.
  • Testing reagents and platforms: Illumina, the world’s largest manufacturer of sequencing instruments and reagents.

HGT’s microbial genetic testing has been applied to categorize Taiwanese microbial groups, and compared with the US published research literature regards of gut microbe categorization, Enterotypes of the human gut microbiome [Nature. 2011 May], Published Literature: Diversity and enterotype in the gut bacterial community of adults in Taiwan. [BMC Genomics Volume 18, Articlenumber: 932 (2017)]

National Taiwan University Food Science Research case. Published literature regarding rat intestinal microbe analysis. Dietary allicin reduces transformation ofL-carnitine to TMAO through impact on gut microbiota [Journal of Functional Foods 15 (2015) 408“417]

research by the City University of Hong Kong which published literature about the analysis of skin flora among the various human race: Insights into the pan-microbiome: skin microbial communities of Chinese individuals differ from other racial groups [Scientific Reports, Published: 16 July 2015]

analyze gut bacteria from obese patients, which further published as: Systematic Analysis of the Association between Gut Flora and Obesity through High-Throughput Sequencing and Bioinformatics Approach [Hindawi Publishing Corporation BioMed Research International Volume 2014]

a study case of the Mackay obstetrics and Gynecology infertility Center for analysis about the correlation between clinical infertility and semen bacteria, which further published as: Bacterial Communities in Semen from Men of Infertile Couples: Metagenomic Sequencing Reveals Relationships of Seminal Microbiota to Semen Quality [PLOS ONE, October 2014]

a research case by the City University of Hong Kong to analyze the bacterial content of the subway station air, which further published as: Indoor-Air Microbiome in an Urban Subway Network: Diversity and Dynamics [Applied and Environmental Microbiology, November 2014]

Testing Process

    Obtain the collection kit

  • The sample collection kit includes: a. A collection rod, b. Stool collection paper (which can be attached to the toilet seat, and can be dissolved in water in the toilet after use), c. Test form and questionnaire, d. Home delivery order

aA collection rod

基因 檢測
保健 益生菌
益生菌 腸胃

bStool collection paper

益生菌 吃

cTest form and questionnaire

改善腸道

dHome delivery order

腸道改善

    Stool collection and inspection (home/clinic)

  • It can be collected and inspected at home or in the medical clinics. After sample collection, the samples are stored in a refrigerated manner and send to the HGT laboratory with the assistance of the medical staff.
益生菌 1

Tear the collection paper and attach it to the toilet seat. Place the feces directly on the collection paper.

康健 益生菌 2

Take the inspection rod out of the package

康健基因 3

Dip the collection rod on the feces 10 times at different locations.

腸道保健 4

Place the collection rod into the storage tube and screw the bottle cap tightly. (DO NOT decant the storage buffer)

腸道 調整 4

Place the collection rod into the storage tube and screw the bottle cap tightly. (DO NOT decant the storage buffer)

調整 體質 5

Remove the collection paper from the toilet and flush it into the toilet.

益生菌 改善 6

Stick the serial number sticker on both storage tube and test form, and place the storage tube into the sealed bag.

糞便檢測
7

Fill in the test form and questionnaire and send them along with the storage tube and test form to the laboratory in a refrigerated manner.

    Obtain the analysis report

  • Receive the analysis report after a month. The paper report contains a physician version (six chapters) and an abstract version (six pages).

    Your personal probiotic formula

  • Distribute suitable probiotic formulas for 30 days or 90 days.
your Probiotics
30 / 90 days
基因 報告
益生菌 健康

The first gut microbe test

腸道 保健

Interpretation of exclusive personal probiotic pairing

益生菌 建議

Perform a second test after 90 days of use

腸胃 調整

Give health advice and adjustment base on the test results.

    Adjust probiotic formula

  • After 30 days or 90 days of using the probiotic formula, collect the sample again, and observe the change in the microbiota. Adjust the probiotic formula accordingly.
Microbiota analysis report - Abstract Version -

Overall Intestinal analysis

Testing Interpretation :
According to the proportion of beneficial and harmful bacteria, constipation, diarrhea, flatulence, and gastrointestinal nutrient absorption capacity, we categorized your microbiota according to the specific categories ( Excessive harmful microbe, gastrointestinal sensitive, functional degradation, nutritional imbalance, daily health maintenance); And provides you an appropriate health plan.
保健 益生菌

Beneficial and harmful microbiota analysis

Testing Interpretation :
According to the proportion of good bacteria: bifidobacterium, lactobacillus etc., Clostridium difficile, Helicobacter pylori bacteria-related disease assessment; constipation, flatulence, diarrhea, colorectal cancer, and allergic condition analysis. Microbiota assessment: biodiversity indicator (more is better), aerobic bacteria index (less is better).
腸道 好菌
消化 益生菌

Functional microbiota analysis

Testing Interpretation :
Akk bacteria ratio : anti-inflammatory, hypoglycemic, hypolipidemic, anti-atherosclerotic, etc.
Recent dietary assessment : evaluate the proportion of lipid-consuming bacteria and meat-consuming bacteria. A high value indicates excessive intake.
Nutritional elements : The intestinal bacteria can metabolize, absorb and synthesize vitamins, metal elements, and essential amino acids.
Calories absorption capacity : This kind of strong absorption capacity means more intake
Microbiota analysis report - Physician Version-

Gut composition

Significance :
The number of species of your gut microbes, the richer the diversity, the better,

Eight indicators of microbiota balance

Significance :
The composition of the microbiota is very complex and there is no certain standard. Microbiota profiling is a relative indicator of health.

Opportunistic and pathogenic bacteria

Significance :
For the detection of opportunistic and pathogenic bacteria in the intestine.

Symbiotic probiotic

Significance :
Check if the amount of probiotics in your intestine is insufficient, average, or sufficient.

Intestines and diseases

Significance :
Based on the detected of microbiota, a risk assessment of bacterial-related diseases is made.

intestinal functional analysis

Significance :
Bacterial plays a role in assisting digestion, absorption, and biosynthesis nutrients in the human body. A balanced microbiota indicates that all functions are in a standard state.

Public Health

gut health risk assessment